Evaluation of Blood Units with Isolated Anti HBC for the Presence of HBV DNA
نویسندگان
چکیده
We screened blood donors in one center in Saudi Arabia for a safety transfusion. We found that among 5043 blood donors negative for HCV and HIV, the incidence of HBsAg positivity was 2.97%. When antiHBc antibody was measured (HBcIg) in HBsAg negative donors, we observed that 21.47% were positive indicating previous exposure to hepatitis B virus. The HBcIg positive blood was further screened for HBsAb and the specimens were found to be reactive in 81.54%. Based on these data blood transfusion was permissible from donors who showed HBsAg negativity, HBcIg positive and HBsAb reactive blood. In order to ensure safety transfusion an aliquot of specimens (n = 80) was further analyzed for HBV DNA by PCR. We found only one specimen positive with incidence of 1.25%. So we recommended restricting transfusion from the previously mentioned donors to emergencies.
منابع مشابه
بررسی عفونت نهفته هپاتیت "ب" در اهداکنندگان خون با HBsAg منفی و anti-HBc مثبت
Introduction: In recent years with introduction of better screening tests, the risk of infection with transfusion-transmitted viruses has been reduced remarkably, although obtaining a zero-risk blood supply still remains international blood transfusion services goal. The routine test for detection of HBV infected blood samples is examination of HBsAg with ELISA method but in occult HBV infectio...
متن کاملIndication of Anti-HBc Antibody Screening and HBV-DNA Detection in Diagnosing Latent Hepatitis B Virus Infection
Background: In spite of available, and sensitive screening assay for detection of hepatitis B virus surface antigen (HBsAg), occasional cases of post-transfusion hepatitis B virus infection are still observed. The aim of the present study was to assess the prevalence of positive anti hepatitis B core (anti-HBc) and presence of HBV-DNA in serum sample of healthy blood donors negative for both HB...
متن کاملPrevalence of Occult Hepatitis B Infection among HBsAg Negative Blood Donors in Golestan Province
Background and Aims: Occult hepatitis B virus infection (OBI) is known as an important source of hepatitis B virus (HBV) infection. It is categorized as Hepatitis B surface antigen (HBsAg) not being present and low DNA viral load in serum. In this study, an attempt was made to investigate the outbreak of anti-HBc and OBI among the HBsAg-negative donors in Golestan province. Materials and Metho...
متن کاملSignificance of Hepatitis B Core Antibody as the Only Marker of Hepatitis B Virus Infection in High Risk Patients
Background and Objective: Presence of hepatitis B core antibody (anti-HBc) in the absence of hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (anti-HBs) is defined, as isolated anti-HBc. little is known about the clinical significance of the isolated anti-HBc in hepatitis B virus (HBV) infections. The aim of this study was to assess the significance of anti-HBc as the onl...
متن کاملDetection of HBV DNA in HBsAg Negative Normal Blood Donors
Background: The risk of infection by transfusion-transmitted viruses has been reduced remarkably. However, a zero-risk blood supply is still desirable. The screening for antibody to HBc (anti-HBc) has been shown as an alternative test for the detection of HBV infection. Objective: The main aim of this study was to evaluate HBV infection markers and the potential value of anti-HBc testing of blo...
متن کاملHEPATITIS B VIRUS INFECTION IN AN ANTI-HBC NEGATIVE PATIENT: A CASE REPORT
ABSTRACT One of the best reliable markers of hepatitis B virus infection is antibodies to the core antigen (Anti-HBc). A first-time blood donor with HBsAg positivity was identified as an HBV carrier that was anti-HBc negative. The patient was followed for 24 months in order to investigate the evolution of his HBV serological profiles and HBVDNA (PCR). In the follow-up for 24 months, HBsAg, HBe...
متن کامل